ISAC PHADIA PDF

The biochip technology takes the form of a miniaturised immunoassay platform where allergen components are immobilised in a microarray. The allergen components are spotted in triplets and covalently immobilised on a polymer coated slide. Each slide contains four microarrays giving results for four different samples per slide. Allergen-bound IgE antibodies are detected by a fluorescence labelled anti-IgE antibody. This educational website, provided by Thermo Fisher Scientific, ImmunoDiagnostics Division, has been produced in partnership with the Institute of Biomedical Science as a pioneering venture to promote excellence in laboratory practice using education and continuing professional development CPD resources.

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Background: Allergic sensitization and diseases have been reported to have a very high and increasing prevalence in elite athletes. The pattern of positivity towards the components tested differed from subject to subject, even in those with the same sensitization to allergen extract SPT or sIgE.

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Melioli G, et al. Clin Biochem. Epub May Goikoetxea MJ, et al. J Investig Allergol Clin Immunol. Determination of multiple allergen-specific IgE by microfluidic immunoassay cartridge in clinical settings.

Shyur SD, et al. Pediatr Allergy Immunol. Epub Dec 9. PMID: Westwood M, et al. Health Technol Assess. ImmunoCAP assays: Pros and cons in allergology. J Allergy Clin Immunol. PMID: Review. Show more similar articles See all similar articles. Cited by 1 article Exercise-induced bronchoconstriction: new evidence in pathogenesis, diagnosis and treatment. Bonini M, Palange P. Bonini M, et al. Asthma Res Pract. Animals Actions. Humans Actions. Phenotype Actions. Skin Tests Actions. Substances Allergens Actions.

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ImmunoCAP ISAC for tricky cases

It is the result of a combination of innovative biochip technology and cutting edge research in molecular allergology. This advanced technology enables measurement of IgE antibodies to a fixed panel of components from 51 allergen sources in a single step, using only 30 ul of serum or plasma. ImmunoCAP ISAC is the first multiplex in vitro diagnostic tool for the allergy specialist that is based exclusively on allergen components. Thermo Scientific: Helping scientists meet the challenges they face in the lab or in the field every day. As in all diagnostic testing, the diagnosis is made by the physican based on both test results and the patient history. ImmunoCAP ISAC — When you need the bigger picture in allergy Trying to find the original cause for allergic reactions can sometimes feel like looking for a needle in a haystack.

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Microarray Evaluation of Specific IgE to Allergen Components in Elite Athletes

Shed light on the real sensitization profile of multisensitized patients. Reveal potential risk for severe food-related reactions. Identify the IgE antibody profile in patients with unsatisfactory response to treatment. Thanks to the cross-reacting proteins ImmunoCAP ISAC can give you information on hundreds of allergen sources in addition to the 51 sources the proteins are derived from. As a result, effective and optimized management can be started earlier, which in turn leads to improved patient health and quality of life.

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ImmunoCAP ISAC Xplain – result guidance when you need it

It is an add-on software which delivers evidence-based result guiding comments on the result report to:. Is the molecule stable and therefore prone to elicit reactions also to heated food? What type of reaction is the molecule commonly associated with: no asymptomatic , local or systemic reactions? The ISAC Xplain knowledge base is based on recent literature and has been approved by allergy specialists in more than 10 countries. Thermo Scientific: Helping scientists meet the challenges they face in the lab or in the field every day. As in all diagnostic testing, the diagnosis is made by the physican based on both test results and the patient history.

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